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| Registros recuperados: 66 | |
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| Bastías,Adriana; Almada,Rubén; Rojas,Pamela; Donoso,José Manuel; Hinrichsen,Patricio; Sagredo,Boris. |
| A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172... |
| Tipo: Journal article |
Palavras-chave: Development; In vitro culture; Hase change; Rejuvenation. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009 |
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| Sasamori,Márcio Hisayuki; Endres Júnior,Delio; Droste,Annette. |
| ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients)) on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Atlantic Forest; Carbohydrate; Conservation; In vitro culture; Orchids; Propagation. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300292 |
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| Castro,Ana Hortência Fonsêca; Braga,Karina de Queiroz; Sousa,Fernanda Marinez de; Coimbra,Mairon César; Chagas,Rafael César Russo. |
| ABSTRACT This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 µM) and BAP (0; 4.44; 8.88; 17.75 µM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; Medicinal plant; In vitro culture; Secondary metabolites. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000100143 |
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| Galdiano Júnior,Renato Fernandes; Mantovani,Cibele; Lemos,Eliana Gertrudes de Macedo. |
| ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro culture; Native orchid; Glucose; Sucrose; Acclimatization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203 |
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| Moreira,André Luís; Silva,Adriano Bortolotti da; Santos,Aline; Reis,Caroline Oliveira dos; Landgraf,Paulo Roberto Correa. |
| The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioreactor; Temporary immersion; Liquid medium; Natural ventilation; In vitro culture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000012 |
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| Wang,Zhengguang; Fu,Chunquan; Yu,Songdong. |
| The present study examined the effects of green tea polyphenols (GTP), insulin-like growth factor-I (IGF-I) and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC) were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa) media supplemented with one of the following supplements: GTP (0, 10, 15 and 20 µM), IGF-I (0, 50, 100 and 150 ng/mL) or glucose (0, 1.5, 5.6 and 20 mM) for 24 h. The results showed that oocytes cultured in media supplemented with 15 µM GTP, 100 ng/mL IGF-I and 5.6 mM glucose, in separate experiments, have higher cleavage and blastocyst rates compared with oocytes cultured in media without or with other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blastocyst quality; Embryo development; In vitro culture; In vitro maturation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200006 |
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| Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa. |
| ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206 |
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| LEDO, A. da S.; LAMEIRA, O. A.; MENEZES, I. C. de. |
| O objetivo do presente trabalho foi estudar as diferentes respostas morfogenéticas de embriões zigóticos de açaizeiro (Euterpe oleracea Mart.) submetidos a várias condições de cultura in vitro. Os experimentos foram conduzidos em laboratório com material vegetal coletado de plantas de açaí na Embrapa Amazônia Oriental, Belém, PA, Brasil. Verificou-se a expressão de um modelo de embriogênese somática, direto, repetitivo a assincronizado em embriões zigóticos maduros cultivados em meio primário MS suplementado com 339.36 ? M de 2,4-diclorofenoxiacético (2,4-D) e transferidos para meio secundário MS na presença de 0,537 ? M de ácido 1-naftalenoacético (ANA) e 12,30 ? M de 2-isopenteniladenina (2iP). A conversão de embriões somáticos em plântulas foi alcançada... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Açaí de touceira; Embrapa Amazônia Oriental; Belém (PA); Pará; Eastern Amazon; Amazônia Oriental; Micropropagación.; Cultivo in vitro; Fitomejoramiento; Embriogénesis; Melhoramento genético vegetal; Açaí; Euterpe oleracea; Cultura in vitro; Embriogênese.; Micropropagação; Embryogenesis.; Micropropagation; Plant breeding; In vitro culture. |
| Ano: 2002 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/496030 |
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| Souza Canada,E. D; Beck,E. |
| An effective tissue culture is an indispensable basis for the production of transgenic plants. In this study, we investigated the induction of embryogenic callus production from the scutellum of spring wheat and subsequent regeneration of plants. Twenty-two Argentine spring wheat varieties from three breeders were screened to select promising genotypes for the optimization of the procedure. Experimental variables were the developmental stage of the immature embryos and the concentration of the growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D). Two developmental stages, termed H and W, respectively (according to He et al., 1986), were selected, and their performance in culture media containing high and low concentrations of 2,4-D was examined.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro culture; Immature embryos; Embryogenic scutellar callus; Callus proliferation. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300006 |
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| Registros recuperados: 66 | |
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